IRMS Laboratory

Expertise and instrumentation to explore stable isotope distributions in soil and aquatic environments.

Service Application

 

About

The SoWa Stable Isotope Facility is located in České Budějovice, Czechia within the framework of the Soil & Water Research Infrastructure, Biology Center, Czech Academy of Sciences.

We are primarily a collaborative service facility that aims to enable and develop stable isotope applications and to answer congruent research questions. We support interdisciplinary investigations of soil and aquatic habitats by offering scientific consultation, student training, and a broad range of instrumentation and approaches for determining stable isotopic ratios of carbon, nitrogen, sulfur, oxygen, and hydrogen. Our commitment is to scientific merit and publication of the stable isotope ratio measurements generated by our combined thought, time, resources, and facilities.

Initiated in February 2018, the facility currently provides the following measurements:

  • Simultaneous δ13C, δ15N, δ 34S solids
  • δ2H and δ18O of solids, alcohols
  • δ13C and δ18O carbonates
  • δ2H, δ18O water
  • δ13C and δ2H methane and C2-6 gases
  • δ13C and δ18O of atmosphere CO2
  • δ13C, δ18O, δ2H fatty acid methyl esters, n-alkanes, and other biomarkers
  • δ13C, δ15N, δ18O, δ2H amino acids

Analysis protocols currently in development include:

  • δ18O, δ17O dissolved O2
  • δ15N and δ18O of nitrate and nitrite
  • δ15N and δ18O of N2 and N2O
  • δ13C, δ18O, δ2H monosaccharides
  • δ13C organic acids

 

Services & Capabilities

Analyte δ13C δ15N δ 34S δ18O δ2H
Solids
Alcohols
Carbonates
Water
C1-6 gases
Atmospheric CO2
Dissolved O2
N2 , N2O, NO2 , NO3
n-alkanes & lipids
Amino acids
Monosaccharides
Organic acids
DOM

 

 

Instrumentation

The SoWa Stable Isotope Facility is equipped with a Thermo ScientificTM 253 PlusTM 10 kV IRMS configured for determining the stable isotopic ratios of C, N, S, O, and H introduced as CO2, N2, SO2, H2, CO, or N2O via continuous flow from the peripheral instruments listed below.

  • SmartEA Isolink (Thermo ScientificTM) converts solid and liquid samples to CO2, N2, SO2, H2, and/or CO gases via flash combustion and, in a single injection, allows for determination of δ 13C, δ 15N, δ34S in samples with C:S ratios up to 103. A high temperature combustion furnace is also available for δ 2H and δ 18O measurements.
  • Trace GC 1310 Isolink II with Conflo-IV and tandem ISQ (Thermo ScientificTM) for separation of ≥C1 gases and combustion/pyrolysis for determination of stable isotopic composition. Analytes may also be channeled to the ISQ for simultaneous generation of MS2 spectra to support compound identification. The GC injection ports are equipped with both standard and PTV injectors as well as a double-shot pyrolyzer.
  • GasBench II (Thermo ScientificTM) with cold trap allows for pre-concentration, purification, and separation of CO2, N2, SO2, H2, CO, and N2O introduced via autosampler injection of headspace samples as well as direct injection onto sample loops (50-250 µL).
  • LC IsoLink for separation and conversion of organic compounds to CO2 for IRMS analysis. The LC flow may be split to an ICPMS/MS detector, allowing for simultaneous analysis of stable isotopic and metal compositions. LC & IC column chromatography is envisioned to allow separation of targeted compound groups (i.e., mono- and oligo-saccharides, sugar alcohols, amino acids and organic acids) as well as non-targeted mixtures of complex natural substances with molecular weights ranging up to 1000 kDa.

 

Projects

Ongoing and completed projects, grants, & collaborations

Collaborator Institution Project Funding Source Status
Gerrit Angst Biology Centre CAS Effects of simulated wildfires on organic matter Ongoing
Gerrit Angst Biology Centre CAS Disentangling the effect of substrate quality on the formation and chemical composition of mineral-protected soil organic matter GAČR 19-00533Y Ongoing
Hana Šantrůčková & Jolanta Niedzwiecka Univ. South Bohemia Carbon use efficiency of 13C-glucose under oxic and anoxic conditions in forest soil Ongoing
Lihini Aluwihare Univ. California San Diego Microbial antioxidants as biomarkers of recalcitrant organic matter CAS MSM200961904 Ongoing
Andreas Lorke Universität Koblenz-Landau The effect of flow velocity on methane production and oxidation in aquatic sediments Ongoing

 

Calendar

Our planned instrument constellation and analyses

 

People

Name Role Phone Room Email
Dr. Travis B. Meador Head of Laboratory +420 387 775 637 206-NB travis.meador@bc.cas.cz
Mgr. Ljubov Poláková Technician 205-NB ljubov.polakova@bc.cas.cz
Dr. Sofia Semitsoglou Postdoc 205-NB sofia.semitsoglou@bc.cas.cz
Stanislav Jabinski Ph.D. Student 205-NB stanislav.jabinski@bc.cas.cz
IRMS Lab a.k.a. “The Luggage” 240-NB